![]() A prominent example is anti-thymocyte globulin (ATG). 4 In addition, rabbit pAbs have been used in a clinical context. Rabbit pAbs have also been utilized as an important tool for food safety assessments. Rabbit pAbs have been used extensively as analytical tools in biomedical research and especially for immunological techniques, such as immunohistochemistry (IHC), western blotting and flow cytometry. Rabbits of the commonly used New Zealand White strain have ~90% IgG-κ (K1), ~10% IgG-κ (K2) and <1% IgG-λ antibodies. The more frequent κ light chain, K1, contains an additional disulfide bridge that links VL and CL. Notably, rabbits have two κ light chains, K1 and K2. CH1 and CH2 are linked through a flexible hinge region that has the amino-acid sequence APSTCSKPTCP (or APSTCSKPMCP in an allotypic variant) and anchors three disulfide bridges (orange) of the IgG molecule, one for each of the two light- and heavy-chain pairs, and one for the heavy-chain pair. The heavy chain consists of an N-terminal variable domain (VH), also shown with its three CDRs, followed by three constant domains (CH1, CH2 and CH3). The light chain consists of an N-terminal variable domain (VL), shown with its three CDRs, followed by one constant domain (CL). The ~150-kDa rabbit IgG molecule contains two identical κ (white) or λ (light gray) light chains paired with two identical heavy chains (dark gray). Schematic drawing of natural rabbit antibodies in IgG format.
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